Changes in intermediate haemoglobins during methaemoglobin reduction by NADPH-flavin reductase

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Changes in intermediate haemoglobins during methaemoglobin reduction by NADPH-flavin reductase.

The changes in intermediate haemoglobins produced during methaemoglobin reduction by NADPH-flavin reductase were analysed by an isoelectric-focusing method. The alpha 3+ beta 2+ and alpha 2+ beta 3+ valency hybrids were observed as intermediate haemoglobins and changed consecutively with time during the reaction. On the basis of the analyses, the course of methaemoglobin reduction was found to ...

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The time course of haemoglobin autoxidation was studied under various conditions at 37 degrees C, and the changes in oxyhaemoglobin, intermediate haemoglobins and methaemoglobin during the reaction were analysed by isoelectric focusing on Ampholine/polyacrylamide-gel plates. Under various conditions (10 mM-phosphate buffer, 10 mM-phosphate buffer with 0.1 M-phosphate buffer, 10 mM-phosphate buf...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1979

ISSN: 0264-6021

DOI: 10.1042/bj1790227